A Quantitative Luminol-Based Assay for ROS Burst Detection in Potato Leaves in Response to Biotic Stimuli.

Muhammad Awais Zahid Ramesh Raju Vetukuri Erik Andreasson

Published in: Methods in molecular biology (Clifton, N.J.) (2022)

Reactive oxygen species (ROS) are important signaling agents in plants and animals. They are involved in diverse processes, including activation of immune responses to pathogen infection. Biphasic detection of ROS in response to pathogen perception is becoming more popular even in important crops like potato as means of screening different germ plasms and mutants generated by for example CRISPR-Cas9 as well as identifying signaling pathways. Here we describe a detailed protocol for quantifying ROS bursts induced in potato leaf discs in response to a bacterial elicitor and Phytophthora infestans.

Keyphrases

reactive oxygen species
cell death
dna damage
crispr cas
immune response
randomized controlled trial
signaling pathway
loop mediated isothermal amplification
real time pcr
high frequency
high glucose
drug induced
epithelial mesenchymal transition
dendritic cells
single cell