Optical imaging of metabolic dynamics in animals.
Lingyan ShiChaogu ZhengYihui ShenZhixing ChenEdilson S SilveiraLuyuan ZhangMian WeiChang LiuCarmen de Sena-TomasKimara TargoffWei MinPublished in: Nature communications (2018)
Direct visualization of metabolic dynamics in living animals with high spatial and temporal resolution is essential to understanding many biological processes. Here we introduce a platform that combines deuterium oxide (D2O) probing with stimulated Raman scattering (DO-SRS) microscopy to image in situ metabolic activities. Enzymatic incorporation of D2O-derived deuterium into macromolecules generates carbon-deuterium (C-D) bonds, which track biosynthesis in tissues and can be imaged by SRS in situ. Within the broad vibrational spectra of C-D bonds, we discover lipid-, protein-, and DNA-specific Raman shifts and develop spectral unmixing methods to obtain C-D signals with macromolecular selectivity. DO-SRS microscopy enables us to probe de novo lipogenesis in animals, image protein biosynthesis without tissue bias, and simultaneously visualize lipid and protein metabolism and reveal their different dynamics. DO-SRS microscopy, being noninvasive, universally applicable, and cost-effective, can be adapted to a broad range of biological systems to study development, tissue homeostasis, aging, and tumor heterogeneity.
Keyphrases
- single molecule
- high resolution
- high speed
- optical coherence tomography
- high throughput
- label free
- living cells
- deep learning
- protein protein
- amino acid
- single cell
- molecular dynamics simulations
- binding protein
- raman spectroscopy
- cell wall
- type diabetes
- nitric oxide
- adipose tissue
- dna methylation
- photodynamic therapy
- metabolic syndrome
- circulating tumor cells
- skeletal muscle