Establishment of a HEK293 cell line by CRISPR/Cas9-mediated luciferase knock-in to study transcriptional regulation of the human SREBP1 gene.
Zihang LiJunli ZhaoNiaz MuhammadDongyang WangQinwen MaoHaibin XiaPublished in: Biotechnology letters (2018)
We successfully generated a novel luciferase knock-in reporter system, which will be very useful for studying transcriptional regulation of the SREBP1 gene and for screening drugs or chemical molecules that regulate SREBP1 gene expression.