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A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos.

Jih-Jin TsaiWei-Liang LiuPing-Chang LinBo-Yi HuangChing-Yi TsaiPei-Yu Alison LeeYun-Long TsaiPin-Hsing ChouSimon ChungLi-Teh LiuChun-Hong Chen
Published in: PloS one (2019)
With performance comparable to a previously validated system, the fully-automated PCR system allows applications of the pan-DENV reagent as a useful tool near points of need to facilitate easy, fast and effective detection of dengue virus and help mitigate versatile public health challenges in the control and management of dengue disease.
Keyphrases
  • dengue virus
  • sensitive detection
  • loop mediated isothermal amplification
  • public health
  • real time pcr
  • zika virus
  • deep learning
  • aedes aegypti
  • machine learning
  • high throughput
  • quantum dots
  • label free
  • global health