CRISPR/Cas9-mediated gene knockin in the hydroid Hydractinia symbiolongicarpus.
Steven M SandersZhiwei MaJulia M HughesBrooke M RiscoeGregory A GibsonAlan M WatsonHakima FliciUri FrankChristine E SchnitzlerAndreas D BaxevanisMatthew L NicotraPublished in: BMC genomics (2018)
This is the first account of CRISPR/Cas9 mediated knockins in Hydractinia and the first example of the germline transmission of a CRISPR/Cas9 inserted transgene in a cnidarian. The ability to precisely insert exogenous DNA into the Hydractinia genome will enable sophisticated genetic studies and further development of functional genomics tools in this understudied cnidarian model.