Transcriptome Profiling of Mouse Embryonic Fibroblast Spontaneous Immortalization: A Comparative Analysis.
Jocshan Loaiza-MossUrsula BraunMichael LeitgesPublished in: International journal of molecular sciences (2024)
Cell immortalization, a hallmark of cancer development, is a process that cells can undergo on their path to carcinogenesis. Spontaneously immortalized mouse embryonic fibroblasts (MEFs) have been used for decades; however, changes in the global transcriptome during this process have been poorly described. In our research, we characterized the poly-A RNA transcriptome changes after spontaneous immortalization. To this end, differentially expressed genes (DEGs) were screened using DESeq2 and characterized by gene ontology enrichment analysis and protein-protein interaction (PPI) network analysis to identify the potential hub genes. In our study, we identified changes in the expression of genes involved in proliferation regulation, cell adhesion, immune response and transcriptional regulation in immortalized MEFs. In addition, we performed a comparative analysis with previously reported MEF immortalization data, where we propose a predicted gene regulatory network model in immortalized MEFs based on the altered expression of Mapk11 , Cdh1 , Chl1 , Zic1 , Hoxd10 and the novel hub genes Il6 and Itgb2 .
Keyphrases
- genome wide
- network analysis
- single cell
- protein protein
- bioinformatics analysis
- dna methylation
- rna seq
- genome wide identification
- poor prognosis
- cell adhesion
- immune response
- small molecule
- copy number
- signaling pathway
- induced apoptosis
- gene expression
- genome wide analysis
- papillary thyroid
- binding protein
- cell cycle arrest
- cell therapy
- electronic health record
- long non coding rna
- lymph node metastasis
- pi k akt
- dendritic cells
- oxidative stress
- transcription factor
- squamous cell carcinoma
- deep learning
- climate change
- squamous cell