Online photochemical derivatization enables comprehensive mass spectrometric analysis of unsaturated phospholipid isomers.
Wenpeng ZhangDonghui ZhangQinhua ChenJunhan WuZheng OuyangYu XiaPublished in: Nature communications (2019)
Mass spectrometry-based lipidomics is the primary tool for the structural analysis of lipids but the effective localization of carbon-carbon double bonds (C=C) in unsaturated lipids to distinguish C=C location isomers remains challenging. Here, we develop a large-scale lipid analysis platform by coupling online C=C derivatization through the Paternò-Büchi reaction with liquid chromatography-tandem mass spectrometry. This provides rich information on lipid C=C location isomers, revealing C=C locations for more than 200 unsaturated glycerophospholipids in bovine liver among which we identify 55 groups of C=C location isomers. By analyzing tissue samples of patients with breast cancer and type 2 diabetes plasma samples, we find that the ratios of C=C isomers are much less affected by interpersonal variations than their individual abundances, suggesting that isomer ratios may be used for the discovery of lipid biomarkers.
Keyphrases
- liquid chromatography tandem mass spectrometry
- fatty acid
- simultaneous determination
- ms ms
- type diabetes
- mass spectrometry
- solid phase extraction
- high performance liquid chromatography
- liquid chromatography
- health information
- social media
- gas chromatography
- tandem mass spectrometry
- gas chromatography mass spectrometry
- high throughput
- small molecule
- healthcare
- high resolution
- ultra high performance liquid chromatography
- skeletal muscle
- insulin resistance
- ionic liquid
- single cell
- high resolution mass spectrometry