Fused Cells between Human-Adipose-Derived Mesenchymal Stem Cells and Monocytes Keep Stemness Properties and Acquire High Mobility.
Karla Montalbán-HernándezCesar Casado-SánchezJosé Avendaño-OrtizJosé Carlos Casalvilla-DueñasGloria Cristina Bonel PérezJulia Prado-MonteroJaime Valentín-QuirogaRoberto Lozano-RodríguezVerónica Terrón-ArcosFátima Ruiz de la BastidaLaura CórdobaFernando Laso-GarcíaLuke DiekhorstCarlos Del Fresno SánchezEduardo López-CollazoPublished in: International journal of molecular sciences (2022)
Human-adipose-derived mesenchymal stem cells (hADMSCs) are multipotent stem cells which have become of great interest in stem-cell therapy due to their less invasive isolation. However, they have limited migration and short lifespans. Therefore, understanding the mechanisms by which these cells could migrate is of critical importance for regenerative medicine. Methods: Looking for novel alternatives, herein, hADMSCs were isolated from adipose tissue and co-cultured with human monocytes ex vivo. Results: A new fused hybrid entity, a foam hybrid cell (FHC), which was CD90 + CD14 + , resulted from this co-culture and was observed to have enhanced motility, proliferation, immunomodulation properties, and maintained stemness features. Conclusions: Our study demonstrates the generation of a new hybrid cellular population that could provide migration advantages to MSCs, while at the same time maintaining stemness properties.
Keyphrases
- stem cells
- cell therapy
- endothelial cells
- mesenchymal stem cells
- adipose tissue
- induced apoptosis
- epithelial mesenchymal transition
- induced pluripotent stem cells
- cell cycle arrest
- pluripotent stem cells
- signaling pathway
- umbilical cord
- bone marrow
- cell death
- oxidative stress
- single cell
- pseudomonas aeruginosa
- type diabetes
- peripheral blood
- staphylococcus aureus
- cystic fibrosis
- candida albicans