Synergistic effects (adsorption and biodegradation) of Streptomyces hydrogenans immobilization on nano-reed biochar for further application in upflow anaerobic sludge blanket.

Water pollution due to wastewater is a serious issue that needs to be studied as many organic compounds are released from wastewater, affecting the ecosystem. Therefore, appropriate treatment methods should be used to prevent these effects. Phragmites australis biochar immobilized with bacteria was prepared in this study for use as an adsorbent in a pilot-scale up-flow anaerobic sludge blanket (UASB) to remove organic matter from wastewater in a buffalo farm. Combining reed biochar and immobilized Streptomyces hydrogenans introduces a synergistic effect: reed biochar serves as a substrate for microbial colonization and provides a conducive environment for microbial growth while Streptomyces hydrogenans , immobilized on the biochar, enhances the degradation of organic matter through its metabolic activities. Suitable techniques were employed, including infrared spectroscopy (FTIR) to determine the functional groups before and after adsorption, scanning electron microscopy (SEM) to determine the morphology of the composite before and after adsorption, X-ray diffraction (XRD) to examine the mineralogical changes through reflectometry, high-resolution diffraction and Brunauer-Emmett-Teller (BET) analyses to determine the surface area that always carried out by nitrogen adsorption/desorption technique based on the BET isotherm. Two-level factorial design experiments optimized using biochar, immobilized with bacteria, were employed to enhance the UASB performance. Chemical oxygen demand (COD) removal and biogas production were studied as a function of four experimental parameters: biochar dose, buffalo sludge dose, pH, and bacteria type. The buffalo sludge (manure) dose negatively affected the model's performance. The results showed better COD removal with Streptomyces hydrogenans S11 inoculation. The optimum biochar dose, buffalo sludge dose, and pH were 20 g L -1 , 0%, and 7.5, respectively. The COD removal efficiency under these experimental conditions reached 92.70% with a biogas production of 5.0 mL. The experimental results of a validated point from the model were 90.80% for COD removal ratio and 4.80 mL for biogas production at 2 g L -1 biochar dose, 0% buffalo sludge dose, and pH 7.5 using Streptomyces hydrogenans S11 bacteria. A buffalo wastewater (BWW) anaerobic digestion experimental model was best fitted to the data under optimal conditions. This study aligns with the United Nations Sustainable Development Goals (SDGs), specifically SDG 6 (clean water and sanitation) and SDG 12 (responsible consumption and production). The implications of our work extend to large-scale applications, promising a greener and more sustainable future for wastewater treatment.