A copy number variant at the HPDA-D12 locus confers compact plant architecture in cotton.
Gaoxiang JiChengzhen LiangYingfan CaiZhaoe PanZhigang MengYanyan LiYinhua JiaYuchen MiaoXinxin PeiWenfang GongXiaoyang WangQiong GaoZhen PengLiru WangJunling SunXiaoli GengPengpeng WangBaojun ChenPeilin WangTao ZhuShoupu HeZhigang MengXiongming DuPublished in: The New phytologist (2020)
Improving yield is a primary mission for cotton (Gossypium hirsutum) breeders; development of cultivars with suitable architecture for high planting density (HPDA) can increase yield per unit area. We characterized a natural cotton mutant, AiSheng98 (AS98), which exhibits shorter height, shorter branch length, and more acute branch angle than wild-type. A copy number variant at the HPDA locus on Chromosome D12 (HPDA-D12), encoding a dehydration-responsive element-binding (DREB) transcription factor, GhDREB1B, strongly affects plant architecture in the AS98 mutant. We found an association between a tandem duplication of a c. 13.5 kb segment in HPDA-D12 and elevated GhDREB1B expression resulting in the AS98 mutant phenotype. GhDREB1B overexpression confers a significant decrease in plant height and branch length, and reduced branch angle. Our results suggest that fine-tuning GhDREB1B expression may be a viable engineering strategy for modification of plant architecture favorable to high planting density in cotton.
Keyphrases
- copy number
- wild type
- mitochondrial dna
- genome wide
- transcription factor
- poor prognosis
- body mass index
- dna methylation
- binding protein
- high resolution
- liver failure
- cell proliferation
- cell wall
- dna binding
- genome wide identification
- drug induced
- air pollution
- gene expression
- respiratory failure
- extracorporeal membrane oxygenation
- long non coding rna
- genome wide association study
- plant growth