Integrative analysis of bulk and single-cell RNA sequencing reveals the gene expression profile and the critical signaling pathways of type II CPAM.
Fengxia LiZheng TanHongyu ChenYue GaoJie XiaTing HuangLiang LiangJian ZhangXianghong ZhangXucong ShiQiang ChenQiang ShuLan YuPublished in: Cell & bioscience (2024)
In summary, by integrating bulk RNA-seq data of type II CPAM with scRNA-seq data, the gene expression profile and critical signaling pathways such as GPCR signaling and PI3K-AKT signaling pathways were revealed. Abnormally expressed genes in these pathways may disrupt epithelial-mesenchymal transition and contribute to the development of CPAM. Given the effectiveness of prenatal treatments of microcystic CPAM using maternal steroids and maternal betamethasone administration, targeting the genes and signaling pathways involved in the development of CPAM presents a promising therapeutic strategy.
Keyphrases
- single cell
- signaling pathway
- rna seq
- pi k akt
- epithelial mesenchymal transition
- gene expression
- genome wide
- high throughput
- induced apoptosis
- cell cycle arrest
- genome wide identification
- electronic health record
- dna methylation
- birth weight
- pregnant women
- transforming growth factor
- systematic review
- randomized controlled trial
- bioinformatics analysis
- pregnancy outcomes
- machine learning
- artificial intelligence
- weight gain
- deep learning
- drug delivery
- transcription factor