Perturbation-Specific Transcriptional Mapping facilitates unbiased target elucidation of antibiotics.

based on correlations between the expression profiles of new molecules and known perturbations, either small molecule inhibition by known antibiotics or transcriptional repression of essential targets by CRISPRi. By rapidly assigning MOA to three new molecules with anti-pseudomonal activity, we provide proof-of-concept for a rapid, comprehensive, systematic, reference-based approach to MOA prediction, with the potential to transform antibiotic discovery efforts.