ZiYinHuaTan Recipe Inhibits Cell Proliferation and Promotes Apoptosis in Gastric Cancer by Suppressing PI3K/AKT Pathway.
Jiahui YuShangjin SongJianpeng JiaoXuan LiuHuirong ZhuLijuan XiuDa-Zhi SunJianfeng CaiXiao-Qiang YuePublished in: BioMed research international (2020)
In recent years, traditional Chinese medicine has played an important role in the treatment of gastric cancer in China. ZiYinHuaTan (ZYHT) recipe was developed for advanced gastric cancer and had shown its promising value in the clinic. In this study, we explore the effect of ZYHT on gastric cancer in vitro and in vivo. ZYHT can inhibit tumor growth and improve the general condition of mice in subcutaneous transplantation nude mice models of gastric cancer. And ZYHT can also inhibit cell proliferation and blocked the cells in G0/G1 to induce cell apoptosis in HGC27 and MGC803 cells. Then, network pharmacology analysis showed that ZYHT may exert antitumor effect mainly through PI3K/AKT signaling pathway. Furthermore, the expression of PI3K, p-Akt, CyclinD1, and Bcl-2 was detected in vitro and in vivo. The results showed that ZYHT could decrease the expression of PI3K, CyclinD1, and Bcl-2 both in vitro and in vivo. These results suggested that ZYHT could be used as a method for the treatment of developed gastric cancer.
Keyphrases
- pi k akt
- cell cycle arrest
- cell proliferation
- signaling pathway
- induced apoptosis
- cell death
- poor prognosis
- cell cycle
- epithelial mesenchymal transition
- oxidative stress
- type diabetes
- primary care
- squamous cell carcinoma
- mesenchymal stem cells
- bone marrow
- radiation therapy
- replacement therapy
- binding protein
- high fat diet induced
- smoking cessation
- insulin resistance
- adipose tissue