Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein-Barr virus-associated gastric cancer.
Hirofumi OhmuraMamoru ItoKeita UchinoChihiro OkadaShigeki TanishimaYuichi YamadaSeiya MomosakiMasato KomodaMiyuki KuwayamaKyoko YamaguchiYuta OkumuraMichitaka NakanoKenji TsuchihashiTaichi IsobeHiroshi AriyamaHitoshi KusabaYoshinao OdaKoichi AkashiEishi BabaPublished in: FEBS open bio (2019)
Epstein-Barr virus (EBV)-associated gastric cancer (GC) is associated with a high degree of DNA methylation. However, the association between chemotherapy susceptibility and tumor DNA methylation in advanced diseases remains unclear. The comprehensive DNA methylation status of GC cells obtained from an advanced EBV-associated GC (EBVGC) case, in which complete response to S-1 plus cisplatin chemotherapy was achieved, was analyzed using a DNA methylation microarray. We compared DNA methylation of GC cells with public data and identified genes with higher methylation in EBVGC cell lines than in normal gastric cells, and genes in which methylation was increased by EBV. Of these genes, ABCG2, AHNAK2, BCL2, FZD1, and TP73 are associated with published evidence for resistance to 5-fluorouracil and cisplatin. Silencing of these genes may be associated with hypersensitivity to chemotherapy.
Keyphrases
- epstein barr virus
- dna methylation
- genome wide
- diffuse large b cell lymphoma
- induced apoptosis
- gene expression
- cell cycle arrest
- copy number
- locally advanced
- healthcare
- endoplasmic reticulum stress
- oxidative stress
- emergency department
- deep learning
- genome wide identification
- signaling pathway
- randomized controlled trial
- drug induced
- artificial intelligence
- big data
- high resolution
- pi k akt
- adverse drug