lncRNA GMDS-AS1 restrains lung adenocarcinoma progression via recruiting TAF15 protein to stabilize SIRT1 mRNA.
Wei PengJunjie JiangJia FuHuaxin DuanJia WangChaojun DuanPublished in: Epigenomics (2023)
Aim: To explore the roles of GMDS-AS1 in the epithelial-mesenchymal transition (EMT) of lung adenocarcinoma (LUAD). Materials & methods: Cell functions were detected by flow cytometry, cell counting kit-8, wound healing assays and transwell assays. RNA immunoprecipitation and pull-down assays were applied for determining the interaction among GMDA-AS1, TAF15 and SIRT1 . A subcutaneous xenograft model was established. Results: GMDS-AS1 downregulation was associated with poor survival of LUAD patients. GMDS-AS1 repressed malignant phenotypes, tumor growth and EMT in vitro and in vivo . Mechanically, GMDS-AS1 recruited TAF15 protein to stabilize SIRT1 mRNA and thereby deacetylated p65 and reduced the recruitment of p65 to MMP-9 promoter, thus inhibiting MMP-9 expression. Conclusion: GMDS-AS1 represses EMT by recruiting TAF15 protein to stabilize SIRT1 mRNA and deacetylate p65, thus restraining LUAD progression.
Keyphrases
- epithelial mesenchymal transition
- binding protein
- signaling pathway
- flow cytometry
- oxidative stress
- high throughput
- ischemia reperfusion injury
- single cell
- transforming growth factor
- end stage renal disease
- protein protein
- wound healing
- chronic kidney disease
- cell therapy
- poor prognosis
- ejection fraction
- gene expression
- amino acid
- newly diagnosed
- long non coding rna
- dna methylation
- transcription factor
- bone marrow
- prognostic factors
- cell migration
- cell proliferation
- long noncoding rna
- nucleic acid