Emitter-Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions.
Hui OuyangJiaxin XianShuai LuoLvxia ZhangWenwen WangZhifeng FuPublished in: ACS applied materials & interfaces (2021)
A facile, one-step doping protocol was adopted to synthesize Co single atomic site catalysts (SASCs) in UiO-66 metal-organic frameworks. In view of highly uniform active sites of Co-O 6 moieties, the SASCs specifically contribute to catalyzing the generation of a large amount of singlet oxygen instead of superoxide or hydroxyl radicals, which endows Co SASCs with a the remarkable enhancement effect (∼3775 times) on luminol chemiluminescent (CL) emission. Interestingly, monolayer titanium carbide MXenes can drastically quench the CL signal of the Co SASC-boosted luminol reaction by ∼94.6% as highly efficient luminescent absorbents. Furthermore, the emitter-quencher pair of Co SASCs and titanium carbide MXenes was successfully adopted to develop an immunoassay method for cardiac troponin I (cTnI) on an immunochromatographic test strip platform. With a sandwich immunoreaction mode, a titanium carbide MXene-labeled cTnI tracer antibody was captured on the test line of a test strip, which significantly inhibited the CL response of the Co SACs-boosted luminol system. The dynamic range for quantitating cTnI is 1.0-100 pg mL -1 , with a detection limit of 0.33 pg mL -1 (3σ). The test strip was successfully used to detect cTnI in human serum samples collected from cardiopathy patients. This proof-of-principle work manifests both the CL enhancement of SASCs and the quenching behavior of MXenes, which shows the thrilling prospects of combinational usage of the two functionalized nanomaterials for tracking biological recognition events.
Keyphrases
- metal organic framework
- highly efficient
- quantum dots
- end stage renal disease
- newly diagnosed
- randomized controlled trial
- chronic kidney disease
- sensitive detection
- ejection fraction
- high throughput
- hydrogen peroxide
- mass spectrometry
- positron emission tomography
- energy transfer
- high resolution
- reduced graphene oxide
- molecularly imprinted
- pet ct
- loop mediated isothermal amplification