Application of Modified mRNA in Somatic Reprogramming to Pluripotency and Directed Conversion of Cell Fate.
Aline Yen Ling WangPublished in: International journal of molecular sciences (2021)
Modified mRNA (modRNA)-based somatic reprogramming is an effective and safe approach that overcomes the genomic mutation risk caused by viral integrative methods. It has improved the disadvantages of conventional mRNA and has better stability and immunogenicity. The modRNA molecules encoding multiple pluripotent factors have been applied successfully in reprogramming somatic cells such as fibroblasts, mesenchymal stem cells, and amniotic fluid stem cells to generate pluripotent stem cells (iPSCs). Moreover, it also can be directly used in the terminal differentiation of stem cells and fibroblasts into functional therapeutic cells, which exhibit great promise in disease modeling, drug screening, cell transplantation therapy, and regenerative medicine. In this review, we summarized the reprogramming applications of modified mRNA in iPSC generation and therapeutic applications of functionally differentiated cells.
Keyphrases
- stem cells
- induced apoptosis
- cell cycle arrest
- mesenchymal stem cells
- cell therapy
- cell fate
- copy number
- endoplasmic reticulum stress
- oxidative stress
- binding protein
- sars cov
- umbilical cord
- emergency department
- signaling pathway
- artificial intelligence
- pluripotent stem cells
- deep learning
- big data
- induced pluripotent stem cells
- pi k akt
- adverse drug