Lignans from Machilus thunbergii as Thymic Stromal Lymphopoietin Inhibitors.
Hyeji ShinYoo Kyong HanYoungjoo ByunYoung Ho JeonKi Yong LeePublished in: Molecules (Basel, Switzerland) (2021)
Thymic stromal lymphopoietin (TSLP) plays an important role in the pathophysiology of various allergic diseases that are mediated by T helper cell type-2 (Th2) responses, including asthma and atopic dermatitis. The primary focus of this study was the identification of potent inhibitors of the TSLP signaling pathway for potential therapeutic use. The 80% methanol extract of Machilus thunbergii bark significantly inhibited the signal transducer and activator of transcription 5 (STAT5) phosphorylation in human mast cell (HMC)-1 cells. Through activity-guided isolation, three lignans (1-3) were obtained and identified as (+)-galbelgin (1), meso-dihydroguaiaretic acid (2), and machilin A (3). Among them, two lignans (1 and 2) significantly inhibited STAT5 phosphorylation and TSLP/TSLPR interaction, as determined by ELISA. Our results indicated that lignans isolated from M. thunbergii are a promising resource for the treatment of allergic diseases.
Keyphrases
- atopic dermatitis
- induced apoptosis
- signaling pathway
- allergic rhinitis
- bone marrow
- cell proliferation
- endothelial cells
- cell cycle arrest
- pi k akt
- oxidative stress
- chronic obstructive pulmonary disease
- protein kinase
- anti inflammatory
- regulatory t cells
- lung function
- epithelial mesenchymal transition
- pluripotent stem cells
- combination therapy
- cystic fibrosis