A Remixed-Fermentation Technique for the Simultaneous Bioconversion of Corncob C6 and C5 Sugars to Probiotic Bacillus subtilis.

The probiotic strain of Bacillus subtilis presents a promising application potential for the value-added bio-utilization of lignocellulosic carbohydrates. By the combined acidolysis pretreatment and enzymatic hydrolysis, hemicellulose and cellulose constituents of corncob were efficiently converted respectively into fermentable C5 and C6 sugars, mainly including xylose and glucose. B. subtilis grew well in xylose solution while it was hindered completely in the acidolysis broth because of the bio-toxicity of degraded chemicals derived from corncob. A mixed-fermentation technique was therefore developed and performed to blend the acidolysis broth and enzymatic hydrolysis slurry together, by which C5 and C6 sugar molecules were successfully fermented and efficiently utilized for the growth of B. subtilis cells with a yield of 0.33 g cells/g sugar consumed. A net amount of 205.9 ± 9.0 g of B. subtilis powder was obtained from 1000 g of corncob that could improve the economic benefits of the process to around 5-7 times.