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Design of a minimal di-nickel hydrogenase peptide.

Jennifer TimmDouglas H PikeJoshua A ManciniAlexei M TyryshkinSaroj PoudelJan A SiessPaul M MolinaroJames J McCannKate M WaldieRonald L KoderPaul G FalkowskiVikas Nanda
Published in: Science advances (2023)
Ancestral metabolic processes involve the reversible oxidation of molecular hydrogen by hydrogenase. Extant hydrogenase enzymes are complex, comprising hundreds of amino acids and multiple cofactors. We designed a 13-amino acid nickel-binding peptide capable of robustly producing molecular hydrogen from protons under a wide variety of conditions. The peptide forms a di-nickel cluster structurally analogous to a Ni-Fe cluster in [NiFe] hydrogenase and the Ni-Ni cluster in acetyl-CoA synthase, two ancient, extant proteins central to metabolism. These experimental results demonstrate that modern enzymes, despite their enormous complexity, likely evolved from simple peptide precursors on early Earth.
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