Enrichment and characterization of extracellular vesicles from ex vivo one-sided human placenta perfusion.
Rachel R ZabelChristin BärJinlu JiRowena SchultzMartin HammerTanja GrotenEkkehard SchleussnerDiana M Morales-PrietoUdo R MarkertRodolfo R FavaroPublished in: American journal of reproductive immunology (New York, N.Y. : 1989) (2020)
Enriched EV populations showed characteristic features of sEVs and lEVs. EV uptake and transfer of miRNAs to recipient cells demonstrated their functional integrity. Therefore, we advocate the ex vivo one-sided placenta perfusion as a robust approach for the collection of placental EVs.