Antibiofilm Potential of Alpha-Amylase from a Marine Bacterium, Pantoea agglomerans .
Charu GoelChippu ShakirAzene TsefayeKuzhunellil Raghavanpillai SabuIdhayadhulla AkbarAseer ManilalMelat WoldemariamNayana VijayanShabna ShahPublished in: The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale (2022)
Bacterial biofilms are a big menace to industries and the environment and also in the health sector, accumulation of which is a major challenge. Despite intensive efforts to curb this issue, a definitive solution is yet to be achieved. Enzyme-templated disruption of the extracellular matrix of biofilm and its control and elimination are emerging as an efficient and greener strategy. The study describes the antibiofilm potential of alpha-amylase from the marine microorganism Pantoea agglomerans PCI05, against food-borne pathogens. Amylase exhibited stability in a wide pH range and retained 50% of its activity at temperatures as high as 100°C. Thermal analysis of the enzyme produced showed thermal stability, up to 130°C. From these findings, it can be envisaged that the alpha-amylase produced from P. agglomerans can be used for starch liquefaction; it was also evaluated for antibiofilm activity. Amylase from this marine bacterium was found to efficiently disrupt the preformed biofilms of food-borne pathogens such as Bacillus cereus , Serratia marcescens , Vibrio parahaemolyticus , Listeria monocytogenes , and Salmonella enterica enterica serotype Typhi based on the value of biofilm inhibitory concentrations.
Keyphrases
- candida albicans
- extracellular matrix
- listeria monocytogenes
- human health
- pseudomonas aeruginosa
- biofilm formation
- staphylococcus aureus
- antimicrobial resistance
- public health
- gram negative
- healthcare
- acute coronary syndrome
- mental health
- risk assessment
- percutaneous coronary intervention
- health information
- radiation therapy
- squamous cell carcinoma
- cystic fibrosis
- left ventricular
- zika virus
- bacillus subtilis
- coronary artery bypass grafting
- lactic acid
- plant growth