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Elevated CO 2 Alters the Physiological and Transcriptome Responses of Pinus densiflora to Long-Term CO 2 Exposure.

Tae-Lim KimHyemin LimHoyong ChungKarpagam VeerappanChangyoung Oh
Published in: Plants (Basel, Switzerland) (2022)
Physiological response and transcriptome changes were observed to investigate the effects on the growth, metabolism and genetic changes of Pinus densiflora grown for a long time in an environment with an elevated atmospheric CO 2 concentration. Pine trees were grown at ambient (400 ppm) and elevated (560 ppm and 720 ppm) CO 2 concentrations for 10 years in open-top chambers. The content of nonstructural carbohydrates was significantly increased in elevated CO 2 . It was notable that the contents of chlorophylls significantly decreased at an elevated CO 2 . The activities of antioxidants were significantly increased at an elevated CO 2 concentration of 720 ppm. We analyzed the differences in the transcriptomes of Pinus densiflora at ambient and elevated CO 2 concentrations and elucidated the functions of the differentially expressed genes (DEGs). RNA-Seq analysis identified 2415 and 4462 DEGs between an ambient and elevated CO 2 concentrations of 560 ppm and 720 ppm, respectively. Genes related to glycolysis/gluconeogenesis and starch/sucrose metabolism were unchanged or decreased at an elevated CO 2 concentration of 560 ppm and tended to increase at an elevated CO 2 concentration of 720 ppm. It was confirmed that the expression levels of genes related to photosynthesis and antioxidants were increased at an elevated CO 2 concentration of 720 ppm.
Keyphrases
  • rna seq
  • genome wide
  • single cell
  • air pollution
  • particulate matter
  • gene expression
  • dna methylation
  • poor prognosis
  • genome wide identification
  • genome wide analysis