Multi-Targeting Nano-Systems Targeting Heterogeneous Cancer Cells for Therapeutics and Biomarker Detection.
Xiao-He RenDi HanXiao-Yan HeTao GuoXue-Si ChenXuan PangSi-Xue ChengPublished in: Advanced healthcare materials (2022)
Cancer heterogeneity plays a vital part in cancer resistance and metastasis. To provide a reliable approach to exert a therapy action and evaluate its efficiency in heterogeneous cancer cells, a multiple targeting delivery vector composed of histone encapsulating the therapeutic or diagnostic agent, hyaluronic acid targeting CD44 overexpressed in stem tumor cells, SYL3C aptamer targeting epithelial cell adhesion molecule (EpCAM) overexpressed in epithelial cancer cells, and CL4 aptamer targeting epidermal growth factor receptor (EGFR) overexpressed in mesenchymal cancer cells, is developed. The vector can efficiently target different cancer cells and circulating tumor cells (CTCs) in the peripheral blood of patients for mucin 1 (MUC1) knockout. Furthermore, the multiple targeting vector can be used to co-encapsulate three types of molecular beacons for probing various mRNA biomarkers at single-cell resolution after genome editing. This study provides an efficient approach for exerting therapeutic actions in heterogeneous cancer cells and assessing the therapeutic efficacy by detection of cancer biomarkers via liquid biopsy.
Keyphrases
- circulating tumor cells
- epidermal growth factor receptor
- cancer therapy
- papillary thyroid
- single cell
- cell adhesion
- hyaluronic acid
- small cell lung cancer
- genome editing
- crispr cas
- peripheral blood
- tyrosine kinase
- stem cells
- gene expression
- dna methylation
- newly diagnosed
- high throughput
- small molecule
- prognostic factors
- ionic liquid
- young adults
- advanced non small cell lung cancer
- single molecule
- lymph node metastasis
- patient reported outcomes
- cell therapy
- patient reported
- magnetic nanoparticles