Exposure to acetaminophen impairs vasodilation, increases oxidative stress and changes arterial morphology of rats.
Hellen Karine Paes PortoMarcella Daruge GrandoLeandra Naira Zambelli RamalhoMarize Campos ValadaresLusiane Maria BendhackAline Carvalho BatistaMatheus Lavorenti RochaPublished in: Archives of toxicology (2019)
Acetaminophen (APAP) is one of the most widely consumed drugs in the world. Studies have shown renal and hepatic damage as the direct result of high oxidative stress induced by APAP. Since the cardiovascular system is sensitive to oxidative stress and literature describes increased cardiovascular dysfunction in APAP consumers, this work aimed to evaluate harmful effects of APAP on the vascular system. Rats were exposed to APAP (400 mg/kg/day in drinking water) for 14 days. Plasma and aortas were collected and stored in - 80 °C and a selection of arteries was prepared for isometric tension recordings, morphological, immunohistochemical and protein expression analysis. The APAP-treated group presented increased transaminases (ALT/AST) and malondialdehyde levels in the plasma compared to controls. Lipid peroxidation, glutathione reductase and superoxide dismutase levels were increased in the plasma and arteries of the APAP group. Nevertheless, glutathione level was reduced as compared to control group. The vasodilation response to acetylcholine and sodium nitroprusside (0.1 nM to 10 µM) was also impaired after APAP treatment; however, the vascular relaxation was restored after treatment with vitamin C (100 µM). Arteries from the APAP group presented reduced wall thickness, collagen deposition, elastic fibers and increased immunoreactivity to nitrotyrosine. eNOS and sGC protein expression remained unchanged and were at similar levels as controls. These findings showed higher oxidative stress and impaired vasodilation in rats exposed to APAP. Furthermore, arteries presented reduced cell layers, collagen, elastin deposition and significantly increased immunoreactivity to nitrotyrosine after APAP treatment.
Keyphrases
- oxidative stress
- drinking water
- dna damage
- diabetic rats
- ischemia reperfusion injury
- endothelial cells
- nitric oxide
- health risk assessment
- cell proliferation
- heavy metals
- signaling pathway
- cell therapy
- single molecule
- resistance training
- amino acid
- fatty acid
- room temperature
- heat stress
- heat shock protein
- solar cells
- protein protein