Application of CRISPR/Cas9-based mutant enrichment technique to improve the clinical sensitivity of plasma EGFR testing in patients with non-small cell lung cancer.

Boyeon KimYoonjung KimSaeam ShinSeung-Tae LeeJae Yong ChoKyung-A Lee

Published in: Cancer cell international (2022)

CRISPR-CPPC is a useful mutant enrichment tool for the sensitive detection of target mutation. When tested in patients with progressive disease, the diagnostic performance of CRISPR-CPPC assay is exceptionally better than that of any other currently available methods.

Keyphrases

crispr cas
sensitive detection
genome editing
quantum dots
wild type
small cell lung cancer
multiple sclerosis
epidermal growth factor receptor
tyrosine kinase
genome wide
high throughput
loop mediated isothermal amplification