Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19.
Tianyi LiScott D SoelbergZachary TaylorVigneshwar SakthivelpathiClement E FurlongJong-Hoon KimSang-Gyeun AhnPeter D HanLea M StaritaJia ZhuJae-Hyun ChungPublished in: Biosensors (2022)
Current point-of-care (POC) screening of Coronavirus disease 2019 (COVID-19) requires further improvements to achieve highly sensitive, rapid, and inexpensive detection. Here we describe an immunoresistive sensor on a polyethylene terephthalate (PET) film for simple, inexpensive, and highly sensitive COVID-19 screening. The sensor is composed of single-walled carbon nanotubes (SWCNTs) functionalized with monoclonal antibodies that bind to the spike protein of SARS-CoV-2. Silver electrodes are silkscreen-printed on SWCNTs to reduce contact resistance. We determine the SARS-CoV-2 status via the resistance ratio of control- and SARS-CoV-2 sensor electrodes. A combined measurement of two adjacent sensors enhances the sensitivity and specificity of the detection protocol. The lower limit of detection (LLD) of the SWCNT assay is 350 genome equivalents/mL. The developed SWCNT sensor shows 100% sensitivity and 90% specificity in clinical sample testing. Further, our device adds benefits of a small form factor, simple operation, low power requirement, and low assay cost. This highly sensitive film sensor will facilitate rapid COVID-19 screening and expedite the development of POC screening platforms.
Keyphrases
- sars cov
- coronavirus disease
- respiratory syndrome coronavirus
- loop mediated isothermal amplification
- label free
- randomized controlled trial
- molecularly imprinted
- computed tomography
- high throughput
- reduced graphene oxide
- fluorescent probe
- small molecule
- room temperature
- dna methylation
- gene expression
- genome wide
- mass spectrometry
- pet ct
- high resolution
- single molecule
- carbon nanotubes