A simple and highly efficient method for multi-allelic CRISPR-Cas9 editing in primary cell cultures.
Pia HoellerbauerMegan KufeldSonali AroraHua-Jun WuHeather M FeldmanPatrick J PaddisonPublished in: Cancer reports (Hoboken, N.J.) (2020)
Our data suggest that this relatively simple method can be used for highly efficient and fast gene knockout, as well as for targeted genomic deletions, even in hyperdiploid cells (such as GSCs). This represents an extremely useful tool for the cancer research community when wishing to inactivate not only coding genes, but also non-coding RNAs, UTRs, enhancers, and promoters. This method can be readily applied to diverse cell types by varying the nucleofection conditions.
Keyphrases
- highly efficient
- crispr cas
- genome editing
- single cell
- cell therapy
- genome wide
- induced apoptosis
- healthcare
- copy number
- mental health
- papillary thyroid
- squamous cell carcinoma
- gene expression
- dna methylation
- cell cycle arrest
- cancer therapy
- machine learning
- transcription factor
- artificial intelligence
- cell proliferation
- lymph node metastasis
- deep learning
- genome wide analysis