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CRISPR/Cas9-mediated precise genome modification by a long ssDNA template in zebrafish.

Haipeng BaiLijun LiuKe AnXiaochan LuMichael HarrisonYanqiu ZhaoRuibin YanZhijie LuSong LiShuo LinFang LiangHaipeng Bai
Published in: BMC genomics (2020)
In summary, these results show that zLOST is a useful method of zebrafish genome editing, particularly for generating desired mutations by targeted DNA knock-in through HDR.
Keyphrases
  • genome editing
  • crispr cas
  • circulating tumor
  • single molecule
  • cancer therapy
  • cell free
  • mass spectrometry
  • gene expression
  • drug delivery
  • high resolution
  • nucleic acid
  • simultaneous determination