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An information-theoretic approach to single cell sequencing analysis.

Michael J CaseyJörg FliegeRubén J Sánchez-GarcíaBen D MacArthur
Published in: BMC bioinformatics (2023)
Thus, our definition of gene heterogeneity leads to a biologically meaningful notion of cell type, as groups of cells that are statistically equivalent with respect to their patterns of gene expression. Our measure of heterogeneity, and its decomposition into inter- and intra-cluster, is non-parametric, intrinsic, unbiased, and requires no additional assumptions about expression patterns. Based on this theory, we develop an efficient method for the automatic unsupervised clustering of cells from sc-Seq data, and provide an R package implementation.
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