Zealpeptaibolin, an 11-mer cytotoxic peptaibol group with 3 Aib-Pro motifs isolated from Trichoderma sp. RK10-F026.
Mira Syahfriena Amir RawaToshihiko NogawaAkiko OkanoYushi FutamuraHabibah A WahabHiroyuki OsadaPublished in: The Journal of antibiotics (2021)
Six new 11-mer peptaibols designed as zealpeptaibolins, A - F were isolated from the soil fungus, Trichoderma sp. RK10-F026, based on the principal component analysis of the MS data from five different culture compositions. Previously, 20-mer peptaibols from the same fungal strain were identified; 11-mer peptaibols in contrast were discovered from a different culture condition, signifying peptaibol production was culture condition-dependent. These peptaibols contained three Aib-Pro motifs in the sequence. The structures were established by NMR and HR-MS experiments including detailed MS/MS fragmentations. The absolute configurations were determined by Marfey's analysis. Zealpeptaibolin F exhibited the strongest cytotoxicity toward K562 leukemia cells with an IC50 value of 0.91 µM.
Keyphrases
- ms ms
- mass spectrometry
- magnetic resonance
- multiple sclerosis
- high resolution
- induced apoptosis
- cell cycle arrest
- bone marrow
- liquid chromatography tandem mass spectrometry
- acute myeloid leukemia
- electronic health record
- cell death
- machine learning
- high performance liquid chromatography
- oxidative stress
- cell proliferation
- liquid chromatography
- solid state
- artificial intelligence
- simultaneous determination
- plant growth
- high resolution mass spectrometry