Simultaneous Light-Triggered Release of Nitric Oxide and Carbon Monoxide from a Lipid-Coated Upconversion Nanosystem Inhibits Colon Tumor Growth.
Yaw Opoku-DamoahRun ZhangHang Thu TaZhi Ping Gordon XuPublished in: ACS applied materials & interfaces (2023)
Gas therapy has gained noteworthy attention in biomedical research, with the rise of gas-releasing molecules enhancing their therapeutic potential, especially when integrated into nano-based drug delivery systems. Herein, we present a lipid-coated gas delivery system to simultaneously shuttle two gas-releasing molecules carrying nitric oxide (NO) and carbon monoxide (CO), respectively. Upconversion nanoparticles (UCNPs) are designed to generate photons at 360 nm upon 808 nm of near-infrared (NIR) irradiation. These in situ-generated UV photons trigger simultaneous NO and CO release from S -nitrosoglutathione (GSNO) and the CO-releasing molecule (CORM), respectively, which are coloaded into lipid-coated UCNP/GSNO/CORM/FA nanoparticles (LUGCF). LUGCF with a GSNO/CORM mass ratio of 2:1 is determined to be optimal in terms of synergistically instigating apoptosis in HCT116 and CT26 colon cancer cells, where both NO/CO are released and subsequent production of ROS are detected. This CO/NO combination nanoplatform exhibits a very effective inhibition of colon tumor growth in vivo at relatively low doses upon a mild 808 nm irradiation. Overall, we effectively integrated two therapeutic gas-releasing molecules in one NIR-responsive nanosystem, presenting a promising therapeutic strategy for future biomedical applications in dual-gas cancer therapy.
Keyphrases
- photodynamic therapy
- nitric oxide
- cancer therapy
- room temperature
- carbon dioxide
- fluorescence imaging
- oxidative stress
- drug delivery
- computed tomography
- cell death
- fatty acid
- stem cells
- working memory
- magnetic resonance
- cell proliferation
- reactive oxygen species
- cell cycle arrest
- endoplasmic reticulum stress
- case report
- fluorescent probe
- radiation induced
- contrast enhanced
- positron emission tomography
- quantum dots
- light emitting