A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering.
Jiuyuan BaiYao LuoXin WangShi LiMei LuoMeng YinYuanli ZuoGuolin LiJunyu YaoHua YangMingdi ZhangWei WeiMaolin WangRui WangChun-Hai FanYun ZhaoPublished in: Nature communications (2020)
Reporter systems are routinely used in plant genetic engineering and functional genomics research. Most such plant reporter systems cause accumulation of foreign proteins. Here, we demonstrate a protein-independent reporter system, 3WJ-4 × Bro, based on a fluorescent RNA aptamer. Via transient expression assays in both Escherichia coli and Nicotiana benthamiana, we show that 3WJ-4 × Bro is suitable for transgene identification and as an mRNA reporter for expression pattern analysis. Following stable transformation in Arabidopsis thaliana, 3WJ-4 × Bro co-segregates and co-expresses with target transcripts and is stably inherited through multiple generations. Further, 3WJ-4 × Bro can be used to visualize virus-mediated RNA delivery in plants. This study demonstrates a protein-independent reporter system that can be used for transgene identification and in vivo dynamic analysis of mRNA.
Keyphrases
- crispr cas
- binding protein
- escherichia coli
- arabidopsis thaliana
- poor prognosis
- gold nanoparticles
- protein protein
- quantum dots
- label free
- amino acid
- genome wide
- high throughput
- gene expression
- copy number
- cystic fibrosis
- nucleic acid
- pseudomonas aeruginosa
- cell wall
- staphylococcus aureus
- bioinformatics analysis
- multidrug resistant
- biofilm formation
- blood brain barrier
- single molecule
- subarachnoid hemorrhage