3D Spheroid Formation Using BMP-Loaded Microparticles Enhances Odontoblastic Differentiation of Human Dental Pulp Stem Cells.
Tae-Jun MinMin Ji KimKyung-Jung KangYeoung Jo JeoungSe Heang OhYoung-Joo JangPublished in: Stem cells international (2021)
Human dental pulp stem cells (hDPSCs) are the primary cells responsible for dentin regeneration. Typically, in order to allow for odontoblastic differentiation, hDPSCs are cultured over weeks with differentiation-inducing factors in a typical monolayered culture. However, monolayered cultures have significant drawbacks including inconsistent differentiation efficiency, require a higher BMP concentration than should be necessary, and require periodic treatment with BMPs for weeks to see results. To solve these problems, we developed a 3D-cell spheroid culture system for odontoblastic differentiation using microparticles with leaf-stacked structure (LSS), which allow for the sustained release of BMPs and adequate supply of oxygen in cell spheroids. BMPs were continuously released and maintained an effective concentration over 37 days. hDPSCs in the spheroid maintained their viability for 5 weeks, and the odontoblastic differentiation efficiency was increased significantly compared to monolayered cells. Finally, dentin-related features were detected in the spheroids containing BMPs-loaded microparticles after 5 weeks, suggesting that these hDPSC-LSS spheroids might be useful for dentin tissue regeneration.
Keyphrases
- stem cells
- endothelial cells
- cell therapy
- induced apoptosis
- drug delivery
- single cell
- mesenchymal stem cells
- cell cycle arrest
- gestational age
- mental health
- cancer therapy
- induced pluripotent stem cells
- endoplasmic reticulum stress
- bone marrow
- cell death
- cell proliferation
- pluripotent stem cells
- combination therapy
- smoking cessation