Lineage Contribution of PDGFRα-Expressing Cells in the Developing Mouse Eye.
Deyi ZhuoYumei DiaoXiaoqi LiYifei HuangLiqiang WangPublished in: BioMed research international (2021)
PDGFRα signaling is critically important in ocular development. Previous data on PDGFRα lacks an expression map with high spatial and temporal resolution and lineage information. In this study, we aim to present a detailed PDGFRα expression and lineage map from early embryogenesis to adulthood. PDGFRα-CreER; mT/mG reporter mice were analyzed. mEGFP-positive cells contributed to multiple ocular lineages in a spatiotemporally regulated manner. A dynamic PDGFRα expression was identified in corneal stromal cells, lens epithelial cells, lens fiber cells, and retinal astrocytes during the entire period of eye development, while PDGFRα expression in retinal astrocytes from E17.5 onwards and in Müller glial cells was identified within two weeks after birth. By revealing detailed characterization of gene expression and function, we present a comprehensive map of PDGFRα-expressing cells in the eye for a better understanding of PDGFRα signaling's role during eye development.
Keyphrases
- induced apoptosis
- cell cycle arrest
- gene expression
- poor prognosis
- endoplasmic reticulum stress
- optical coherence tomography
- spinal cord injury
- signaling pathway
- dna methylation
- machine learning
- healthcare
- cell death
- metabolic syndrome
- oxidative stress
- adipose tissue
- crispr cas
- skeletal muscle
- insulin resistance
- spinal cord
- depressive symptoms
- artificial intelligence
- social media
- deep learning