Anti-Colitic Effects of Ethanol Extract of Persea americana Mill. through Suppression of Pro-Inflammatory Mediators via NF-κB/STAT3 Inactivation in Dextran Sulfate Sodium-Induced Colitis Mice.
Joo Young HongKyung-Sook ChungJi-Sun ShinGeonha ParkYoung Pyo JangKyung Tae LeePublished in: International journal of molecular sciences (2019)
Persea americana Mill, cv. Hass, also known as avocado, has been reported to possess hypolipidemic, anti-diabetic, anti-oxidant, cardioprotective, and photoprotective potencies. However, few studies have reported its anti-colitic effects. In this study, we investigated anti-colitic effects of ethanol extract of P. americana (EEP) in dextran sulfate sodium (DSS)-induced colitic mice and the involved molecular mechanisms. EEP effectively improved clinical signs and histological characteristics of DSS-induced colitis mice. In DSS-exposed colonic tissues, EEP reduced expression levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokines such as interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α. Moreover, EEP suppressed DSS-induced activation of nuclear factor-kappa B (NF-κB) and signal transducer and activator of transcription 3 (STAT3). Consistent with in vivo results, EEP also suppressed protein and mRNA expression levels of iNOS, COX-2, and pro-inflammatory cytokines via NF-κB and STAT3 inactivation in LPS-induced RAW 264.7 macrophages. Taken together, our data indicate that ethanol extract of avocado may be used as a promising therapeutic against inflammatory bowel diseases by suppressing the NF-κB and STAT3 signaling pathway.
Keyphrases
- nuclear factor
- lps induced
- signaling pathway
- nitric oxide synthase
- toll like receptor
- oxidative stress
- inflammatory response
- pi k akt
- anti inflammatory
- cell proliferation
- nitric oxide
- rheumatoid arthritis
- diabetic rats
- high fat diet induced
- type diabetes
- poor prognosis
- high glucose
- adipose tissue
- deep learning
- skeletal muscle
- drug induced
- endothelial cells
- high resolution
- long non coding rna
- wild type
- endoplasmic reticulum stress