Mechanism of Juglone-Induced Cell Cycle Arrest and Apoptosis in Ishikawa Human Endometrial Cancer Cells.
Yuan-Yuan ZhangFan ZhangYing-Shuo ZhangKiran ThakurJian-Guo ZhangYun LiuHuan KanZhao-Jun WeiPublished in: Journal of agricultural and food chemistry (2019)
The molecular mechanism of Juglone-induced cell cycle arrest and apoptosis in human endometrial cancer cells was investigated. Juglone was purified from the green husk of Carya cathayensis Sarg and identified by HPLC, LC-MS/MS, and NMR. At an IC50 of 20.81 μM, juglone significantly inhibited Ishikawa cell proliferation, as shown by S phase arrest mediated by inactivation of cyclin A protein ( p < 0.05). The ROS levels increased significantly after exposure to juglone, which paralleled increases in the mRNA and protein expression of p21 and decreases in the levels of CDK2, cdc25A, CHK1, and cyclin A. The expression of Bcl-2 and Bcl-xL was significantly down-regulated, whereas the expression of Bax, Bad and cyto c was up-regulated, and we later confirmed the involvement of the mitochondrial pathway in juglone-induced apoptosis. Our in vitro results stated that juglone can be studied further as an effective natural anticancer agent.
Keyphrases
- cell cycle arrest
- cell death
- pi k akt
- induced apoptosis
- cell cycle
- signaling pathway
- cell proliferation
- endothelial cells
- oxidative stress
- high glucose
- poor prognosis
- endoplasmic reticulum stress
- binding protein
- diabetic rats
- transcription factor
- induced pluripotent stem cells
- endometrial cancer
- magnetic resonance
- mass spectrometry
- reactive oxygen species
- atomic force microscopy
- simultaneous determination
- long non coding rna