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KAT2A and KAT2B prevent double-stranded RNA accumulation and interferon signaling to maintain intestinal stem cell renewal.

Mai-Uyen NguyenJahangir IqbalSarah PotgieterWinston HuangJulie PfefferSean WooCaifeng ZhaoMatthew A LawlorRichard YangRahma RizlyAngela HalsteadSharon Y R DentJosé B SáenzHaiyan ZhengZuo-Fei YuanSimone SidoliChristopher E EllisonMichael P Verzi
Published in: Science advances (2024)
Histone acetyltransferases KAT2A and KAT2B are paralogs highly expressed in the intestinal epithelium, but their functions are not well understood. In this study, double knockout of murine Kat2 genes in the intestinal epithelium was lethal, resulting in robust activation of interferon signaling and interferon-associated phenotypes including the loss of intestinal stem cells. Use of pharmacological agents and sterile organoid cultures indicated a cell-intrinsic double-stranded RNA trigger for interferon signaling. Acetyl-proteomics and sequencing of immunoprecipitated double-stranded RNA were used to interrogate the mechanism behind this response, which identified mitochondria-encoded double-stranded RNA as the source of intrinsic interferon signaling. Kat2a and Kat2b therefore play an essential role in regulating mitochondrial functions and maintaining intestinal health.
Keyphrases
  • stem cells
  • dendritic cells
  • nucleic acid
  • binding protein
  • healthcare
  • public health
  • single cell
  • mental health
  • dna methylation
  • gene expression
  • immune response
  • cell death
  • climate change