Osteosarcoma (OS) is a common primary malignant bone tumor, and it is necessary to further investigate the molecular mechanism of OS progression. The expression of kinetochore associated protein 1 (KNTC1) and minichromosome maintenance 2 (MCM2) was detected by immunohistochemistry, quantitative PCR (qPCR) and Western blot. Gene knockdown or overexpression cell models were constructed and the proliferation, apoptosis, cell cycle and migration were detected in vitro, besides, xenograft models were established to explore the effects of KNTC1 downregulation in vivo. Public databased and bioinformatics analysis were performed to screen the downstream molecules and determine the expression of MCM2 in cancers. KNTC1 was overexpressed in OS tissues and positively correlated with overall survival of OS patients. KNTC1 knockdown inhibited the proliferation and migration, and arrested G2 phase, and induced apoptosis. Besides, KNTC1 downregulation restricted the xenograft tumor formation. MCM2, one of the coexpressed genes, was highly expressed in sarcoma and downregulated after KNTC1 knockdown. MCM2 overexpression heightened the proliferation and migration ability of OS cells, which was reversed the inhibiting effects of KNTC1 knockdown. KNTC1 was overexpressed in OS and promoted the progression of OS by upregulating MCM2.
Keyphrases
- induced apoptosis
- signaling pathway
- endoplasmic reticulum stress
- cell proliferation
- cell cycle
- oxidative stress
- cell cycle arrest
- poor prognosis
- bioinformatics analysis
- end stage renal disease
- pi k akt
- gene expression
- chronic kidney disease
- healthcare
- ejection fraction
- mental health
- cell death
- newly diagnosed
- emergency department
- binding protein
- prognostic factors
- stem cells
- south africa
- high throughput
- cell therapy
- single cell
- mass spectrometry
- genome wide identification
- electronic health record
- genome wide analysis