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CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes.

Bastiaan EversKatarzyna JastrzebskiJeroen P M HeijmansWipawadee GrernrumRoderick L BeijersbergenRené Bernards
Published in: Nature biotechnology (2016)
High-throughput genetic screens have become essential tools for studying a wide variety of biological processes. Here we experimentally compare systems based on clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) or its transcriptionally repressive variant, CRISPR-interference (CRISPRi), with a traditional short hairpin RNA (shRNA)-based system for performing lethality screens. We find that the CRISPR technology performed best, with low noise, minimal off-target effects and consistent activity across reagents.
Keyphrases
  • genome wide
  • genome editing
  • crispr cas
  • dna methylation
  • high throughput
  • copy number
  • gene expression
  • single cell