Pneumococcal Extracellular Vesicles Mediate Horizontal Gene Transfer via the Transformation Machinery.
Sarah Werner LassShaw CamphireBailey E SmithRory A EutseyJojo A PrenticeSaigopalakrishna S YerneniAshni ArunAndrew A BridgesJason W RoschJames F ConwayPhil CampbellN Luisa HillerPublished in: bioRxiv : the preprint server for biology (2023)
Bacterial cells secrete extracellular vesicles (EVs), the function of which is a matter of intense investigation. Here, we show that the EVs secreted by the human pathogen Streptococcus pneumoniae (pneumococcus) are associated with bacterial DNA on their surface and can deliver this DNA to the transformation machinery of competent cells. These findings suggest that EVs contribute to gene transfer in Gram-positive bacteria, and in doing so, may promote the spread of drug resistance genes in the population.
Keyphrases
- induced apoptosis
- genome wide
- cell cycle arrest
- genome wide identification
- circulating tumor
- endothelial cells
- single molecule
- cell free
- endoplasmic reticulum stress
- oxidative stress
- signaling pathway
- gene expression
- dna methylation
- gram negative
- pi k akt
- induced pluripotent stem cells
- electron transfer
- pluripotent stem cells