Gold Nanoparticles Inhibit Extravasation of Canine Osteosarcoma Cells in the Ex Ovo Chicken Embryo Chorioallantoic Membrane Model.
Anna MałekMarek WojnickiAleksandra BorkowskaMichał WójcikGabriela ZiółekRoman LechowskiKatarzyna Zabielska-KoczywąsPublished in: International journal of molecular sciences (2023)
Canine osteosarcoma (OS) is an aggressive bone tumor with high metastatic potential and poor prognosis, mainly due to metastatic disease. Nanomedicine-based agents can be used to improve both primary and metastatic tumor treatment. Recently, gold nanoparticles were shown to inhibit different stages of the metastatic cascade in various human cancers. Here, we assessed the potential inhibitory effect of the glutathione-stabilized gold nanoparticles (Au-GSH NPs) on canine OS cells extravasation, utilizing the ex ovo chick embryo chorioallantoic membrane (CAM) model. The calculation of cells extravasation rates was performed using wide-field fluorescent microscopy. Transmission electron microscopy and Microwave Plasma Atomic Emission Spectroscopy revealed Au-GSH NPs absorption by OS cells. We demonstrated that Au-GSH NPs are non-toxic and significantly inhibit canine OS cells extravasation rates, regardless of their aggressiveness phenotype. The results indicate that Au-GSH NPs can act as a possible anti metastatic agent for OS treatment. Furthermore, the implemented CAM model may be used as a valuable preclinical platform in veterinary medicine, such as testing anti-metastatic agents.
Keyphrases
- gold nanoparticles
- induced apoptosis
- small cell lung cancer
- squamous cell carcinoma
- cell cycle arrest
- poor prognosis
- reduced graphene oxide
- endoplasmic reticulum stress
- endothelial cells
- oxidative stress
- stem cells
- sensitive detection
- drug delivery
- fluorescent probe
- single molecule
- mesenchymal stem cells
- signaling pathway
- high throughput
- young adults
- cell proliferation
- combination therapy
- mass spectrometry
- pi k akt
- living cells
- quantum dots
- climate change