Towards measuring growth rates of pathogens during infections by D2 O-labeling lipidomics.
Cajetan NeubauerAlex L SessionsIan R BoothBenjamin P BowenSebastian H KopfDianne K NewmanNathan F DalleskaPublished in: Rapid communications in mass spectrometry : RCM (2018)
This study demonstrates how trace level measurement of stable isotopes in intact lipids can be used to quantify lipid metabolism in pure cultures and provides guidelines that enable growth rate measurements in microbiome samples after incubation with a low percentage of D2 O.