Identification of antibacterial peptides from endophytic microbiome.
Mysore V TejesviP PicartM KajulaH HautajärviL RuddockH H KristensenA TossiH G SahlS EkS MattilaA M PirttiläPublished in: Applied microbiology and biotechnology (2016)
Endophytes, microorganisms living inside plant tissues, are promising producers of lead compounds for the pharmaceutical industry. However, the majority of endophytes are unculturable and therefore inaccessible for functional studies. To evaluate genetic resources of endophytes, we analyzed the biodiversity of fungal microbiome of black crowberry (Empetrum nigrum L.) by next-generation sequencing and found that it consists mainly of unknown taxa. We then separated the host and the endophyte genomes and constructed a fosmid expression library from the endophytic DNA. This library was screened for antibacterial activity against Staphylococcus aureus. A unique antibacterial clone was selected for further analysis, and a gene En-AP1 was identified with no similarity to known sequences. The expressed, folded protein En-AP1 was not active against S. aureus, while tryptic digests exhibited antimicrobial activity. Seven out of twelve synthesized peptides, predicted antibacterial in silico, exhibited in vitro activity towards both S. aureus and Escherichia coli. We propose that the En-AP1 protein is degraded in the library host E. coli and antimicrobial fragments are released from the cell, explaining the in vitro antibacterial activity of the clone. This is the first report of a novel gene expressed in vitro derived from an endophytic microbiome, demonstrating the potential of finding novel genes and compounds from unculturable endophytes.
Keyphrases
- silver nanoparticles
- genome wide
- copy number
- staphylococcus aureus
- escherichia coli
- transcription factor
- genome wide identification
- amino acid
- circulating tumor
- binding protein
- dna methylation
- poor prognosis
- protein protein
- biofilm formation
- gene expression
- single cell
- anti inflammatory
- bioinformatics analysis
- wastewater treatment
- cell free
- genome wide analysis
- small molecule
- single molecule
- molecular docking
- mesenchymal stem cells
- klebsiella pneumoniae
- cystic fibrosis
- human health
- case control
- wound healing
- pseudomonas aeruginosa
- methicillin resistant staphylococcus aureus
- multidrug resistant