A ratiometric fluorescent probe for reversible monitoring of endogenous SO2/formaldehyde in cytoplasm and nucleoli regions and its applications in living mice.
Yanyan MaWenjie GaoLinlin ZhuYuping ZhaoWeiying LinPublished in: The Analyst (2020)
The excessive production of sulfur dioxide (SO2) and formaldehyde (FA) by organisms is closely associated with several diseases, including neurological disorders, respiratory disease, and genotoxicity. RNA plays a central role in the expression of genes. However, it is difficult for fluorescent probes to explore the intersecting correlation of SO2 and FA near the nucleolus regions due to the protective obstacle of the cell nucleus membrane. Herein, a unique fluorescent probe (DP) for reversible detection of the interaction of SO2 and FA in the nucleolus regions was constructed. In light of the outstanding attributes of DP, including a large fluorescence ratio variation, ultrafast detection of SO2, and excellent reversibility, it was successfully employed to track the variation of endogenous SO2 and FA in the cytoplasm and nucleolus regions of live cells. In addition, the DP probe could be applied to the imaging of endogenous SO2 and then recovered by FA in live mice.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- induced apoptosis
- poor prognosis
- label free
- room temperature
- high resolution
- stem cells
- genome wide
- gene expression
- cell cycle arrest
- oxidative stress
- cell death
- physical activity
- dna methylation
- mass spectrometry
- mesenchymal stem cells
- gram negative
- insulin resistance
- bone marrow
- hydrogen peroxide
- signaling pathway
- cell proliferation
- ionic liquid