Gene Expression Profile in Peripheral Blood Nuclear Cells of Small Ruminant Lentivirus-Seropositive and Seronegative Dairy Goats in Their First Lactation.
Joanna Pławińska-CzarnakAlicja MajewskaJoanna ZarzyńskaJanusz BogdanJarosław KabaKrzysztof AnuszEmilia BagnickaPublished in: Animals : an open access journal from MDPI (2021)
The immune response to a viral antigen causes inflammatory cell infiltration to the tissue, which creates a suitable environment for the replication of the virus in macrophages, and the recruitment of more monocytes to the site of infection, or latently infected monocytes. The aim of the study was to analyze the transcriptomic profile of peripheral blood nuclear cells isolated from SRLV-seropositive and SRLV-negative goats at the peak of their first lactation. SRLV-seropositive goats were probably infected via colostrum. Custom transcriptomic microarrays for goats were designed and developed, namely the Capra hircus gene expression array, which features ~50,000 unique transcripts per microarray. Only four genes were differentially expressed, with up-regulated expression of the GIMAP2, SSC5D and SETX genes, and down-regulated expression of the GPR37 gene in SRLV-seropositive vs. SRLV-seronegative goats. However, in an RT-qPCR analysis, the result for the SETX gene was not confirmed. The differences in the expressions of the studied genes indicate an active inflammatory process in the SRLV-seropositive goats at the early stage of infection.
Keyphrases
- peripheral blood
- gene expression
- genome wide
- genome wide identification
- induced apoptosis
- dna methylation
- early stage
- poor prognosis
- single cell
- transcription factor
- human milk
- cell cycle arrest
- oxidative stress
- bioinformatics analysis
- genome wide analysis
- copy number
- squamous cell carcinoma
- dendritic cells
- binding protein
- endoplasmic reticulum stress
- signaling pathway
- mass spectrometry
- neoadjuvant chemotherapy