Image-guided transplantation of single cells in the bone marrow of live animals.
Raphaël TurcotteClemens AltJudith M RunnelsKyoko ItoJuwell W WuWalid ZaherLuke J MortensenLev SilbersteinDaniel C CôtéAndrew L KungKeisuke ItoCharles P LinPublished in: Scientific reports (2017)
Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into the bone marrow of live mice. We developed an optical platform that integrates a multiphoton microscope with a laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation. These tools allow image-guided single cell transplantation with high spatial control. The platform was used to deliver single hematopoietic stem cells. The engraftment of transplants was tracked over time, illustrating that the technique can be useful for studying both normal and malignant stem cells in vivo.
Keyphrases
- bone marrow
- cell therapy
- stem cells
- single cell
- mesenchymal stem cells
- induced apoptosis
- hematopoietic stem cell
- high throughput
- cell cycle arrest
- rna seq
- high resolution
- high speed
- randomized controlled trial
- endoplasmic reticulum stress
- type diabetes
- high dose
- low dose
- oxidative stress
- mass spectrometry
- ultrasound guided
- signaling pathway
- adipose tissue