Antiproliferative and apoptotic potential of methotrexate lipid nanoparticles in a murine breast cancer model.

Chaitali Surve Ananya Banerjee Anupriya S Rajasree Chakraborty Dhiraj Kumar Ramesh Butti Mahadeo Gorain Sabyasachi Parida Gopal C KunduSupriya ShidhayeSrinivas Patnaik

Published in: Nanomedicine (London, England) (2022)

Aim: To evaluate the efficacy of novel methotrexate-loaded nanoparticles (MTX-NPs) in vitro and in vivo in the treatment of breast cancer. Materials & methods: MTX-NPs were tested for cellular uptake, cell viability, cell cycle, cellular wound migration and changes in tumor volume using characterized NPs. Results: The solid lipid NPs (SLNPs) showed strong cellular uptake, increased apoptosis, controlled cytotoxicity at lower IC 50 of methotrexate and a sizable reduction in tumor burden. Conclusion: MTX-NP oral formulation can be a promising candidate in breast cancer treatment with improved cellular uptake and in vivo efficacy.

Keyphrases

cell cycle
high dose
cell death
drug delivery
cell proliferation
oxide nanoparticles
oxidative stress
fatty acid
endoplasmic reticulum stress
cancer therapy
low dose
anti inflammatory
risk assessment
young adults
human health
childhood cancer
surgical site infection