Interleukin-6 increases adrenal androgen release by regulating the expression of steroidogenic proteins in NCI-H295R cells.
Xian WeiJunfeng ZhangWei PengHao XuZhewen WeiLinhao PangJihong LiuTao WangPublished in: Journal of cellular physiology (2020)
The purpose of this study was to investigate the potential mechanism of interleukin-6 (IL-6) on the stimulation of excessive androgen secretion in human NCI-H295R adrenocortical cells. We performed transcriptome sequencing of cancer and paracancerous tissues obtained from functional adrenal cortical adenomas. The secretion of dehydroepiandrosterone sulfate (DHEAS) in NCI-H295R cells was detected by a chemiluminescence assay. The expression of messenger RNA (mRNA) was detected by real-time polymerase chain reaction and that of protein was detected by western blotting. The expression of secretogranin II (SCG2) and IL-6 were significantly increased in cancer tissues. Upregulation of mRNA and protein levels of AKR1C3, CYP11A, CYP17A1, 3βHSD, and SULT2A1 was observed after stimulation with IL-6. IL-6 could also increase the expression of StAR mRNA and proteins. Our results suggest that IL-6 can promote androgen secretion by regulating the expression of genes related to androgen pathways.
Keyphrases
- poor prognosis
- binding protein
- induced apoptosis
- cell cycle arrest
- gene expression
- long non coding rna
- papillary thyroid
- signaling pathway
- genome wide
- endothelial cells
- cell proliferation
- oxidative stress
- mass spectrometry
- risk assessment
- high throughput
- climate change
- weight gain
- sensitive detection
- simultaneous determination