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Comparison of real-time PCR and the Kato-Katz method for the diagnosis of soil-transmitted helminthiasis and assessment of cure in a randomized controlled trial.

Beatrice BardaChristian SchindlerRahel WampflerShaali AmeSaid M AliJennifer Keiser
Published in: BMC microbiology (2020)
In conclusion, a single real-time PCR is as sensitive as quadruplicate KK for T. trichiura and A. lumbricoides detection but more sensitive for hookworm, which has an influence on the estimated treatment efficacy. PCR method with DNA extraction using the "bead-beating protocol" should be further promoted in endemic areas and laboratories that can afford the needed equipment. The study is registered at ISRCTN (no. 20398469).
Keyphrases
  • real time pcr
  • randomized controlled trial
  • circulating tumor
  • single molecule
  • cell free
  • combination therapy
  • clinical evaluation
  • smoking cessation